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The following thermocycling conditions were used for the PCR: Initial denaturation at 50C for 2 min
Enzyme systems regenerate e.g., pyruvate dehydrogenase (PDH) is activated, lactate production decreases, glycolysis and TCA cycle proceed and energy supply is restored
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eight animals per group were used for the efficacy studies and another eight animals per group were used for the metabolic/indirect calorimetry studies
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