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Cells (3 10 5 /well) were seeded in 6-well plates, then place the plates into the IncuCyte Live-Cell Analysis System (ESSEN BIOSCIENCE)
Cc gc t do c to ra bi nhiu yu t khc nhau, bao gm tip xc vi nh nng mt tri, nhim v thm ch l cng thng
was supported by the National Science Technology Council, Taiwan: NSTC107-2923-M-006-002-MY3 (M-ERA.NET2 nanoPD)
reported in 2015 that treatment of mice with both LC (0.4 mg/mouse) and ALC (0.12 mg/mouse) resulted in increased number of mature oocytes and less degradation of oocytes
TUNEL assay On experimental day P38, pancreas samples were fixed in 2% w/v paraformaldehyde for 2 h and processed for TUNEL assay, which was performed using the One Step TUNEL Apoptosis Assay Kit (Beyotime, Shanghai, China) according to the manufacturers instructions
