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For RNA extraction, TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA) was used to isolate total RNA from both cells

DsbASP was oxidized with a 10-fold molar excess of AT-2 and roGFP2 was reduced with a 10-fold molar excess of DTT for 10 min at 20 C and then the oxidant or reductant were removed using Micro Bio-Spin P-30 gel columns (BioRad, Feldkirchen, Germany, #7326223) or NAP-5 columns (Cytiva, Freiburg, Germany, #17085302) according to the suppliers protocol

Hatzioannou A, Boumpas A, Papadopoulou M, Papafragkos I, Varveri A, Alissafi T, et al

Dispose of unused material, solutions, and contaminated items according to institutional and local chemical/biological waste rules

While the risk of death and suicide attempts is lower during and shortly after pregnancy than in the general female population, suicides account for up to 20% of all postpartum deaths and represent a leading cause of mortality in the peripartum period, which corroborates the high prevalence of suicide risk in our sample of women (24)

Current clinical guidelines no longer recommend aspiration for subcutaneous sites because major blood vessels are not located in the subcutaneous layer