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To measure changes in intra-cellular calcium levels in midbrain GABA neurons during cocaine seeking, a Cre-dependent GCaMP8f virus was infused into the VTA of rats expressing Cre-recombinase under the GAD promoter and a fiberoptic cannula was implanted directly above the infusion site (Fig 5A)

UCK2 (which converts uridine to UMP) in particular requires ATP as a substrate 63 and has been linked with mitochondrial function 64 and mTOR signalling 65

Glutamine promotes muscular hydration by retaining water in muscle cells, which is necessary for electrolyte balance