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To conduct this first pharmacological experiment, effective, high doses of the compounds, validated in preclinical models with metabolic alterations 33,34,35,41,42 , were selected

One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)

5 mg/kg BCTC) was used in this experiment

Healthcare providers are encouraged to educate patients about these risks and guide them toward safe, approved options

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: a systematic review of human studies