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(D) Cells were pretreated with or without GLP-1 (10 nM), LY294002 (20 M) or U0126 (20 M) for 1h, incubated with H 2 O 2 (1 mM) for 12 h, then stained with primary antibodies of LC3 followed by staining with secondary antibodies

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For many people, the additional side effects of 15 mg do not justify the marginal improvement in results