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For a 100mg vial targeting 5mg per dose at 0.25mL injection volume: 100mg 5mg = 20 doses

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After PBS wash 2~3 times, cells were maintained in serum-free RPMI 1640 medium (Gibco, Thermo Fisher Scientific) for the duration of the assay

This includes enlarged hands, feet, jaw, and internal organ enlargement (for example: heart, liver)

& Munday, C

The calculator exists because dose accuracy determines result validity