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Two of the six designs, pro1 and pro6, showed detectable binding to protegrin via changes in binder tryptophan fluorescence, with affinities of 7.2 and 1.2 M, respectively (Figure 3f, Supplementary Figure 22d)

Endocrinology 135, 589594

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Regulation of cholesterologenesis by the oxysterol receptor, LXRalpha

-actin (mouse anti-human -actin, 1:5000, A5441, Sigma) was used as endogenous control