If you are doing this the right way, it is anything but easy
As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only
Ashwagandha also influences monoaminergic systems, but its effects are largely indirect, arising from normalization of hypothalamic-pituitary-adrenal (HPA) axis hyperactivity, modulation of GABAergic tone, and attenuation of stress-induced neurotransmitter imbalance
Retrieved from Eli Lilly and Company-b
International recommendations for the Management of Adults Treated with ketogenic diet therapies
Additionally, by supporting healthy blood sugar levels, it reduces the likelihood of cravings and energy crashes, which are common obstacles in weight loss journeys