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For sorting, mononuclear cells were reacted with anti-CD38 PE and anti-CD138 FITC antibodies and primary MM cells were isolated using fluorescence-activated cell sorting (BD FACSAria II, San Jose, CA, USA)
Van Hinsbergh VW et al
Key steps include: Wash your hands thoroughly and prepare a clean, flat surface
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As the body metabolizes stored glycogen, bound water is released and excreted through the kidneys, potentially increasing urine output temporarily
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