For the double blank samples, 95 l of blank fecal homogenate or urine was added to 905 l of methanol, which was mixed via vortex for 1 min and centrifuged at 20,200 g for 10 min at 4C, respectively
When the body produces too many free radicals, it can cause oxidative stresswhich impacts the body and its cells
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Thus, sufficient quantities of glycine may be just as necessary and rate-limiting as cysteine