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Untreated and BSO-treated HeLa cells, infected with PV1-wt (moi 10) were labeled with 35 S-Translabel, as described above, and the cell lysates were analyzed on a 1530% sucrose gradient for the separation of the 75S and 150S intermediates (Materials and Methods)

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There are some limitations acknowledged to this study, including a relatively low sample size (owing to the rarity of IIH) and a short follow-up period, partially mitigated by the standardized data collection and thorough quality control applied within the VIIH

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In a resting cell, the GSH: GSSG ratio typically exceeds 100:1, while in various models of oxidative stress, this ratio has been found to be as low as 10:1 and in some cases, even 1:1