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Further quantum mechanics/molecular mechanics (QM/MM) calculations showed that the trisulfide cofactor lowers the energy barrier for nucleophilic attack by H 2 S by 6.3 kcal/mol, increasing catalytic efficiency by approximately 105-fold

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All coding sequences for NAPstars, Peredox, HyPer7 and NERNST were codon optimised for expression in Saccharomyces cerevisiae , synthesised and delivered in a pUC57 plasmid (GenScript Biotech, Rijswijk, Netherlands)

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K.MartnekJ.HornekA.KmonckovE.ZdekZ.et al (2009)