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Cell proliferation was evaluated using a Promega CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) as per the manufacturers instructions (Promega G3580)

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Pathogenic variants in genes encoding transporters and channels found in the DCT are associated with several diseases in humans including Bartter syndrome, Gitelman syndrome, familial hyperkalemic hypertension, EAST syndrome ( E pilepsy, A taxia, S ensorineural deafness, and salt-wasting renal T ubulopathy), and hereditary hypomagnesemias

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As you snooze, your brain consolidates and processes the information youve acquired during the day, enabling you to form and retain new memories