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It is worth to mention that available reports describing the encapsulation of GSH- or albumin-protected AuNCs are limited to hydrophilic, rather than hydrophobic, matrices such as nanogels 25 , aqueous core of microcapsules 26 , silica nanoparticles, protein nanoparticles 27,28 , polymer nanoparticles 29,30 and metal-organic frameworks 31

The washed beads were mixed with 0.5 mL (10 fold dilution of initial concentration) purified human recombinant Trx1 protein (0.85 mg), diluted to 8 mL with 50 mM HEPES buffer (pH 7.5) and supplemented with 1 mM TCEP