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Rameau et al., 2007), such as in uncontrolled epilepsy (Sumadewi et al., 2023) and stroke (Qin et al., 2022)

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The researchers concluded that long-term body mass loss is only partially mediated by caloric restriction melanocortin system activation appears to increase energy expenditure independently (Cote et al., 2017)

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SDS-PAGE and western blotting Samples were loaded onto 10% SDS-polyacrylamide gels then transferred to 0.45 m PVDF membranes and blocked with 5% skimmed milk [or 5% bovine serum albumin (BSA) for anti-phospho antibodies] in TBS buffer supplemented with 0.1% Tween 20 (TBS-T)