Brunstrom M, Carlberg B
Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment
Both the YE and R5 LSC subtypes reduced liver injury markers and improved immune balance, with the less mature YE cells exhibiting the greatest efficacy ( Table 2 )
Copper coordination is pH-dependent , avoid strongly acidic reconstitution buffers as they can disrupt the copperpeptide complex
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Apply 15-20 minutes before sun exposure, reapply frequently if needed
