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In order to target roGFP2 into the periplasm, roGFP2 was PCR amplified using the primer pair listed in supplementary table S3 from the pCC_ roGFP2 plasmid as template

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Therefore, PAK1 binding can be used to monitor Rac1 activation status

Gallagher EJ, Sun H, Kornhauser C, Tobin-Hess A, Epstein S, Yakar S, et al

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