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A landmark study that used affinity chromatography with ubiquitinsepharose to separate and reconstitute the E1E2E3 ubiquitin conjugation system, demonstrating that all three components are required for conjugation of ubiquitin to lysine residues in the substrate

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Sikiric P, Seiwerth S, Rucman R, Drmic D, Stupnisek M, Kokot A, et al