Area under the curve (AUC) analysis showed significant decrease in SKO-treated mice, returning to values similar to those observed in wild-type (WT) PBS-treated mice ( Figures 1B, D )
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Preparation of vitrified specimen EM grids (Quantifoil, Grolbichau, Germany, 200 mesh copper R1.2/1.3) were glow discharged for 30 s in high-pressure air using Harrick plasma cleaner (Harrick, Ithaca, NY)
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