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Insert the needle into the pinch at a 4590-degree angle (use 45 degrees if the fat layer is thin) [13] [14]
After packing, the column was washed with buffer containing 20 mM HEPES, pH 7.4, 100 mM NaCl, 10% (v/v) glycerol, 5 M GLP-2, 25 M TCEP, 5 mM MgCl 2 , 1 mM MnCl 2 , 0.1% (w/v) LMNG and 0.02% (w/v) CHS first (10 column volumes), and then with decreased concentrations of detergents 0.03% (w/v) LMNG, 0.01% (w/v) GDN and 0.008% (w/v) CHS (20 column volumes)
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Both ML models demonstrated high cross-validated performance, with AUC values of 0.91 (0.791.00) for sPLS-DA and 0.80 (0.630.97) for Boruta (Fig