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Blood was collected by submandibular puncture into 1 mL Eppendorf tubes, the plasma was separated and stored in 80 C until used
If you experience incomplete dissolution with neutral water, try 0.1% acetic acid (approximately pH 3.5) as your initial diluent
Ravussin E, Sanchez-Delgado G, Martin CK, et al
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However, ongoing monitoring for gallbladder symptoms is essential, and lifestyle modifications should be maintained to support overall digestive health
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