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21.6 L of the TMT label reagents were added to each 20 g of samples and were incubated for 1 hr at RT

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RNA-seq Total RNA was isolated using the RNeasy kit (Qiagen) from three independent replicates of MDA-MB-231 cells incubated for 5 h with ML162 (125 nM) or ESA (100 M) and two replicates from the control incubated with the vehicle for ESA (methanol)