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Jan 22 2019;20(3)doi:10.3390/ijms20030459 Van der Touw T, Andronicos NM, Smart N

In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min

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Moreover, using recombinant YBX1 protein for an in vitro binding assay, we further confirmed that YBX1 directly binds to the 5UTR probe but not scramble negative control probes (Fig

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