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The amplified fragment from gr knockout mutant using primer pairs gr OF/ gr OR was sequenced to confirm the mutants

In lipopolysaccharide primed macrophages, capsaicin induced TRPV1 activation and Ca 2+ influx promote polarization toward an M2 macrophage phenotype characterized by anti-inflammatory properties, thereby improving the adipose tissue inflammatory microenvironment and attenuating insulin resistance (63)

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No accumulation occurs with repeated dosing