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nicotine and flavoring aerosols also drive oxidative stress

however, these same studies recognize the added risk of aspiration and regurgitation with their continuation as well (Ushakumari and Sladen 2024

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Following a further incubation of 15 or 30 min at 37C under 5% CO 2 , 100 L of the reaction medium was transferred to 400 L of ice-cold methanol containing internal standard (IS) to terminate the reaction