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Blood and tissue processing For all studies blood was drawn by cardiac puncture in anesthetized mice and collected in 5 l EDTA (BD medical) before being spun in a microcentrifuge at 9.8 revolutions per minute (RPM) for 5 min at room temperature

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44 Thirdly, retinal NF-B in diabetes is activated at the beginning of retinopathy, initiating proapoptotic procedures, 45 and stay energetic while interacts with reactive oxygen species (ROS) even if the apoptotic process of retinal capillary cells speeds up

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