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Analysis of metabolic data Multivariate statistical analysis, such as principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA), was carried out after normalization of the preprocessed data matrix

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In experimental models for the induction of tissue damage, 20 mg/kg MTX was a commonly used dose for rats (Reference Moodi, Hosseini and Abedini16Reference Azadnasab, Kalantar and Khorsandi20)

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Common clinical manifestations of MC4R variants are summarized in Table 3 [23,34-38]