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Unfortunately, degraded peptide should be discarded

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E3 ligases are grouped into three classes according to their unique domains, including Really Interesting New Gene (RING) or U-box domain-containing E3 ligases, Homologous to E6AP C-terminus (HECT) domain-containing E3 ligases, and RING-between-RING (RBR) domain-containing E3 ligases (Morreale and Walden, 2016)

doi: 10.1021/acs.jafc.1c07568

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Your course won't be convoluted