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According to the 2.4 micrograms (mcg) daily for ages 14 years or older 2.6 mcg daily for pregnant people 2.8 mcg daily for people who are breastfeeding or nursing It may be possible to get enough vitamin B12 from foods

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In individuals with pre-existing gastrointestinal conditions such as inflammatory bowel disease, coeliac disease, or irritable bowel syndrome, introducing any new supplement may temporarily affect bowel habits as the digestive system adapts

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Functional assessment may require mitochondrial respiration, proton-leak measurements, tissue temperature, oxygen consumption, adrenergic responsiveness, cold challenge, or whole-body energy-expenditure measurements