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Bile acid profiling by liquid chromatography-tandem mass spectrometry (LC-MS/MS) Liver samples (30 mg) were homogenized with 200 l pre-cooled ultrapure water, 800 L pre-cooled methanol, and 10 L internal standard, then mixed thoroughly by vortex, followed by incubation at 20 C for 20 min to allow the protein precipitation

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