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Dimerization of substrate adaptors can facilitate cullin-mediated ubiquitylation of proteins by a tethering mechanism: a two-site interaction model for the Nrf2-Keap1 complex

In brief, heart tissue was homogenized in ice-cold 6% perchloric acid, centrifuged at 1000 rpm for 15 min at 0.5 C, and the supernatant fluid was injected into HPLC after neutralization to pH 67

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The results correspond to one representative experiment

Regulatory note: Regulatory status changes