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The cells were collected after treatment, washed twice with cold phosphate-buffered saline, and treated with 1 mL of cold citrate buffer (0.24 M sucrose, 40 mM sodium citrate, pH 7.6)

If you can hold longer, do it

It also helps deliver energy needed for workouts by enhancing mitochondrial energy production and supporting fat metabolism

Thymosin beta4: structure, function, and biological properties supporting current and future clinical applications

For individuals on GLP-1 therapies who are also engaged in a consistent resistance training program, creatine supplementation may offer meaningful benefits

treat it as a baseline, not a bonus