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The present study on the atomistic details of amphetamine binding and binding affinity to the tyramine oxidase along with the comparison with two natural substrates of this enzyme namely tyramine and phenylalanine provides strong evidence for the promiscuity-based metabolism of amphetamine by the tyramine oxidase enzyme of E
For example, the Ellman's assay mentioned earlier exploits the reaction of thiols with DTNB to produce a colored thiolate anion, which can be quantified spectrophotometrically
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Yang et al., 2016)
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