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Craig Primack, M.D
Saturation and diminishing returns: Beyond certain dose, additional peptide provides minimal incremental benefit as receptors become saturated or downstream pathways maximally activated
( in vivo models
Supplementary Validation Method: LCMS/MS For high-sensitivity detection requirements, liquid chromatographytandem mass spectrometry is used for supplementary validation, especially suitable for trace component analysis and metabolic studies
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Methods: After confirming that berberine can lower blood glucose and improve insulin resistance in db/db mice, the identity maintenance, proliferation and apoptosis of islet cells were detected by immunohistochemistry and immunofluorescence
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