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BPA treatment reduced the length of axon regeneration, but axonal fusion rate was not enhanced by any tested concentration of BPA

Superoxide measurements in liver and kidney by electron paramagnetic resonance (EPR) spectroscopy Mitochondrial superoxide in liver and kidney tissue was measured by EPR spectroscopy using a mitochondrial targeted probe, 1-hydroxy-4-[2-triphenylphosphonio)-acetamido]-2,2,6,6-tetramethylpiperidine,1-Hydroxy-2,2,6,6-tetramethyl-4-[2-(triphenylphosphonio)acetamido] piperidinium dichloride (mito-TEMPO-H) (Enzo Life Sciences, Farmingdale, NY) 24,25

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Peptide mass: 10mg Bacteriostatic water: 2mL Concentration: 10mg 2mL = 5 mg/mL = 5,000 mcg/mL Peptide per unit (100-unit syringe): 5 mg 100 = 0.05 mg/unit = 50 mcg/unit If research protocol calls for 250 mcg per research unit: 250 mcg 50 mcg/unit = 5 units on the syringe Total doses per vial at 250 mcg each: 10,000 mcg 250 mcg = 40 research doses That single 10mg vial, reconstituted with 2mL of bacteriostatic water, provides 40 research doses of 250 mcg each, drawn to the 5-unit mark on a standard insulin syringe

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