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CP: Data curation, Investigation, Methodology, Project administration, Validation, Writing original draft
Wilkes, J
Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates
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There was a 1.4-fold decrease for HEK-293 cells and a 1.1-fold decrease for K-562 cells in total glutathione levels at 25M of menadione compared to the vehicle control
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