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In vitro GST assay with CDNB (5) Glutathione transferase assays with CDNB ( 5 ) were performed in 50 mM Tris, pH 7.5 with 5% clarified cell lysate, 1 mM CDNB and 1 mM GSH

It is also possible that radical intermediates nonenzymatically produced from hydroperoxy-phospholipids directly react with GSH, leading to the formation of a highly pro-oxidant GS radical

CuCP could be demetallated by EDTA at non-physiological pH value (pH = 11) in dose-dependent manner, characteristic for fast binding equilibrium

Its also great for individuals exposed to pollution or stress

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Proc Natl Acad Sci U S A 99:350510 Brown GC, Borutaite V (2004) Inhibition of mitochondrial respiratory complex I by nitric oxide, peroxynitrite and S -nitrosothiols