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Following this, the appropriate amount of pre-diluted fluorescent-labeled antibodies (eBioscience, 11-0032-82, 48-0041-82, 11-9668-80

[4] In case of accidental large overdose (e.g., someone receives far more frequent injections than prescribed), monitoring for symptoms like flushing, tingling in extremities, or liver enzyme elevations would be prudent

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This is especially relevant for endpoints such as viability, migration, and tube formation, which can be sensitive to culture conditions and reagent variability