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These lasting effects were linked to epigenetic changes , chemical modifications that influence when and how genes are active

doi: 10.1001/jama.2016.7602 19 Di LorenzoNAntoniouSABatterhamRLBusettoLGodorojaDIossaAet al

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After protein concentrations were detected with a BCA protein assay kit (Boster, catalog AR0146), 5x SDS was added

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