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A 1.5-min baseline of BRET measurement was taken before the addition of ligand and BRET signal was measured at 10-s intervals for further 9 min
This means slower results, inconsistent week-to-week responses, and the frustrating sense that the protocol is not working when the real problem is preparation
Blonde L, Berard L, Saremi A, Huang Y, Aroda VR, Raccah D
R., Lingvay, I., Lutz, K., & Birkenfeld, A
The risk is shortcut thinking
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