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Until the sections were dry, images were captured by transmission electron microscope (HT7700, Hitachi, Tokyo, Japan), and the number of autophagosomes and autophagolysosomes was mounted by Image-proplus 6.0 (Media Cybernetics, Rockville, MD, USA)
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For preparing the parasite cell extract, pellets were diluted in equal volumes of phosphate-buffered saline, and a complete protease inhibitor cocktail (Roche, Mannheim, Germany) was added
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