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ELISA Kit Components Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Assay Procedure Summary Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37C

Depressive-like behavior In male rodents, supplementation with vitamin E (P31-45) [104] or folic acid (P41-61) [41] reversed the POS-induced depressive-like behavior measured in the FST at P6061 [41] and at P45-47 [104], but not in the splash test (ST) at P45-47 [104]

You need two types