Reprogramming microorganisms for the biosynthesis of astaxanthin via metabolic engineering
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Previous studies have indicated high transcriptional similarities between lipid droplet-enriched microglia in Grn / mouse brains and lipid droplet-accumulating microglia observed in aged mice 70
Mice were housed up to 5 per cage at The Centre for Phenogenomics (TCP) specific pathogenfree mouse facility (Mount Sinai Hospital, Toronto, ON, Canada) on a 12-hour light/dark cycle at 23C with ad libitum access to standard rodent chow diet (18% kcal from fat, 2018 Harlan Teklad) and acidified drinking water
A 3ml or 5ml syringe with a long needle works well for most vials