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Cells resuspended in the growth medium (GM, DMEM containing 10% fetal bovine serum (FBS, Life Technologies) and 1% anti-anti), and the single cells were seeded in a cell culture flask

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The authors administered oral Dihexa to APP/PS1 transgenic mice (a standard amyloidopathy model of Alzheimers disease) and showed that brain AngIV levels were elevated relative to wild-type after Dihexa administration, that spatial learning and memory were restored on Morris water maze testing, that hippocampal neuronal density and synaptophysin protein expression were preserved, that astrocyte and microglial activation were reduced, and that pro-inflammatory cytokines (IL-1, TNF-) decreased while anti-inflammatory IL-10 increased

Common side effects include: Nausea and vomiting Diarrhoea or constipation Reduced appetite (which is also a therapeutic effect) Injection site reactions (redness, bruising, or mild discomfort) These effects are typically mild to moderate and tend to resolve as the body adjusts to the medication

Working in a clean environment, using sterile supplies, and preparing your workspace before opening any vial are some of the simplest ways to improve laboratory consistency and reduce contamination risk