Luciferase reporter assays A bicistronic vector pcDNA3 RLuc POLIRES FLuc (empty reporter) (Addgene, 45642) and pcDNA3 RLuc POLIRES FLuc containing human PRKAA1 5UTR upstream of FLuc (5UTR reporter) were transfected into shCtrl and shGDH1 or shYBX1 Huh7 cells using Lipofectamine 2000 (Thermo Fisher)
Besides, many LAB are also used for the industrial or food grade production of biomolecules like vitamins, exopolysaccharides, polyols etc
I starting feeling bad almost straight away after my 2nd glass of wine and this morning I feel like Ive been up all night dancing
Once generated, HBs were maintained in an expansion medium
An unexpected finding emerged suggesting that a single instance of the STRE component (CCCCT) was sufficient to increase the expression of a reporter construct in response to thermal stress
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