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For adipose, snap-frozen iWAT tissue was homogenized using a Dounce tissue homogenizer in 3 volume of 0.25 M sucrose, 2 mM EDTA, 0.1 M KPO 4 , pH 7 buffer containing 1:100 dilution of both phosphatase and protease inhibitors (Millipore, P8340 and P2850)

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Time-series analysis confirmed this significant increase, with semaglutide-related calls increasing by an additional nearly 10% per quarter after the 2021 approval

Expression of Apurinic/Apyrimidinic endonuclease-1 (Ape-1) in h

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Another randomized controlled trial by Zhang et al., 2025 was assessed as low risk of bias, with adequate randomization, outcome completeness, and statistical analysis