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The final measurement at the end of this step reflects the total content of GSH (free GSH + GSSG) in the samples, to calculate the GSSG content, the free GSH content must be subtracted from the total GSH content
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T., Myles, E
Further, ebselen follows the analogous catalytic steps as that of native GPx enzymes, and the activation energies for the ebselen-catalyzed and native enzyme-catalyzed GPx reactions are 55 kJ/mol and 36.5 kJ/mol, respectively [78]
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