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Quantitative RT-PCR RNA was extracted from cell lysates using RNeasy Mini Kit (Qiagen, Chatsworth, CA, USA) and was reverse-transcribed using QuantiTect Reverse Transcription Kit (Qiagen, Chatsworth, CA, USA)

When combined with a tailored low-calorie, low-fat diet and regular exercise, overweight adults including those with high blood pressure, diabetes, or high cholesterol can experience substantial improvements in various health indicators

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Notably, these vesicles influence gut barrier function by modulating mucosal innate immune cells such as macrophages and DCs (328)