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[4] The second activation step takes place predominantly in the kidneys where 1-hydroxylase converts 25-hydroxyvitamin D to 1,25-dihydroxyvitamin D (calcitriol), an active secosteroid hormone that binds nuclear receptors in enterocytes, osteoblasts, renal tubules, parathyroid cells, and numerous immune and endocrine cells to modulate hundreds of genes involved in calcium-phosphate homeostasis, skeletal remodelling, and immunoregulation

This creates an interesting divergence

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o , Application of GLP-1 and GLP-1MK-801 induced changes in fluorescence indicative of rises in intracellular calcium (%F/F 0 ) as shown by these representative fluorescent responses of two different Fura 2-AM loaded cells to application of GLP-1 (1 M) or GLP-1MK-801 (1 M)

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This confirms the peptide has initiated its intended molecular mechanism